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Host colonization differences between citrus and coffee isolates of Xylella fastidiosa in reciprocal inoculation Scientia Agricola
Prado,Simone de Souza; Lopes,João Roberto Spotti; Demétrio,Clarice Garcia Borges; Borgatto,Adriano Ferreti; Almeida,Rodrigo Piacentini Paes de.
Citrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) are important diseases in Brazil associated with closely-related strains of Xylella fastidiosa, but little is know about host aoverlappingnd importance of citrus and coffee as inoculum sources of these strains. In this study, reciprocal-inoculation experiments were performed to determine if CVC and CSA isolates are biologically similar within citrus and coffee plants. These two hosts were mechanically inoculated with a CVC and a CSA isolate of X. fastidiosa at four concentrations ranging between10³ and 10(9) colony forming units CFU mL-1. At two, four and eight months after inoculation, the infection efficiency and bacterial populations of the isolates in each host were determined by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus variegated chlorosis; Coffee leaf scorch; Inoculum concentration; Bacterial population; Epidemiology.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000300005
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Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors Scientia Agricola
Marucci,Rosangela Cristina; Giustolin,Teresinha Augusta; Miranda,Marcelo Pedreira de; Miquelote,Helen; Almeida,Rodrigo Piacentini Paes de; Lopes,João Roberto Spotti.
Rearing leafhopper (Hemiptera: Cicadellidae) vectors free of Xylella fastidiosa is a requirement for studies of various aspects of vector-pathogen interactions. The selection of a plant that allows vector development but not bacterial multiplication is desirable to produce healthy vectors. In this study, two leafhopper hosts, Vernonia condensata ('boldo') and Aloysia virgata ('lixeira') were needle inoculated with citrus and coffee strains of X. fastidiosa to evaluate if these plants support pathogen colonization. The inoculated plants did not present symptoms and the pathogen was not detected by culture and PCR tests, neither soon after inoculation (7-14 days) nor later, at 1, 4, 6 and 12 months after inoculation. To obtain healthy adults of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vernonia condensata; Citrus variegated chlorosis; Coffee leaf scorch; Sharpshooter vectors; Rearing technique.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010
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